Table 5.
Details of 11 chloroplast SSR primers validated in 12 Piper species (21 accessions).
| Primer ID | Primer sequence | Repeat motif | No. of alleles | Annealing temp. (°C) | Allele size (bp) |
|---|---|---|---|---|---|
| BPC1 | f-CGAATACACCAGCTACGCCT r-AGTTTCCGTCTGGGTATGCG |
(CAG)4 | 4 | 55 | 220 |
| BPC2 | f-CTGGGCGCGAGGACTAAAAA r-TGAGTGGACTAAAGCGGCAG |
(GA)4 | 7 | 55 | 280 |
| BPC3 | f-CCCCCAACTCAACTAGGTCG r-GGTATTTCGGCGAGTCCTCA |
(AT)4 | 5 | 55 | 271 |
| BPC4 | f-TCCAAATCAATCCTGCGGGT r-GGCTTGGCCGAAGAACTTCA |
(TC)4 | 5 | 55 | 178 |
| BPC5 | f-TTGGCCCACTCTTCATCGAC r-GTGATGGAGTTTTGTTTCCAGGA |
(TG)5 | 7 | 55 | 231 |
| BPC6 | f-TTCCACTCACCCCATGGTTG r-TCAGATTCAAAACGGCTTGCT |
(AATG)3 | 5 | 55 | 280 |
| BPC7 | f-GTCCTGCGAAGGGAAGGAAA r-ACTTTGGGATTGCTGAATTGCA |
(AG)4 | 4 | 55 | 272 |
| BPC8 | f-GAACTTTTTGGTTTGGGGGCT r-GTGCTGATCGTTACAGGCCT |
(ATAGT)3 | 11 | 55 | 278 |
| BPC9 | f-TGAGTTGGGCGCTTTAACCA r-ACTCCAAATTCGGGGGTCAA |
(GA)4 | 4 | 55 | 273 |
| BPC10 | f-ACGACAGGCAAACCTCTAGA r-GCACCAATCCCGCTTTCTTG |
(TTTA)3 | 4 | 55 | 26 |
| BPC11 | f-CGCCAGACAAAAGTCAGAACA r-ATGCGGTTAAACCAGCTGTG |
(CT)4 | 4 | 55 | 210 |