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. 2023 Jan 12;13:1095879. doi: 10.3389/fimmu.2022.1095879

Figure 2.

Figure 2

Secretion of cytokines and adhesion molecules by CTR (n=8), EO- (n=6) and LO-PE (n=4) HBCs. Multiplex-ELISA-on-beads assay for followed pro-inflammatory and anti-inflammatory cytokines (A) IL-6, (B) IL-12p-70, (C) TNF-α, (D) ICAM, (E) IL-17a, (F) E-selectin, (G) IL-4, (H) IL-10 and (I) IL-13, respectively. Multiplex was performed in duplicates. (J) ELISA against IL-8 performed in duplicates (CTR n=10, EO-PE n=5 and LO-PE n=5). (K) ELISA assay against TGF-β1 performed in duplicates (CTR n=12, EO-PE n=6 and LO-PE n=6). Secretion of respective cytokines was normalized to the total protein content measured in the cell culture supernatants. Statistical significance was tested using ANCOVA with adjustment for gestational age followed by Sidak’s post-hoc test. *p ≤ 0.05, **p ≤0.01.