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Validation experiment of BRD4 expression in cells. (A) Immunofluorescence analysis of BRD4 (green) in normal neuroglial cells and U-251 MG cells (Scale bar: 20 μm). (B) Mean relative fluorescence intensity values for each group. (C) Western blot analysis of BRD4 protein between normal neuroglial cells and U-251 MG cells. β-Actin was an internal control. (D) Relative expression value of BRD4 protein (means ± SD, n = 3,**p < 0.01).
