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. 2023 Jan 10;17(2):896–909. doi: 10.1021/acsnano.2c05286

Figure 1.

Figure 1

Intravenous injection of mitochondria shows no therapeutic effect on mice myocardial IR injury. (A) Schematic representation of the mitochondrial transplantation strategy. (B) Representative transmission electron microscopy (TEM) images of isolated mitochondria from donor mice heart. Scale bar = 1 μm. (C) Representative left ventricular (LV) M-mode echocardiographic tracings. Images are representative of independent mice. (D,E) Statistical analysis of echocardiographic left ventricular ejection fraction (LVEF) and shortening fraction (LVFS). (F) Representative images and (G) statistical analysis of Evan’s blue and TTC staining in IR heart treated with or without mitochondria. (H–J) Western blots and statistical analyses for expression changes of apoptosis proteins cleaved caspase 3, Bax, and Bcl2. (K–N) Western blots and statistical analyses for expression changes of inflammatory factors, including NLRP3, IL6, and cleaved IL 1β. Mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. Statistical analysis was carried out by a one-way ANOVA analysis followed by Tukey’s test for post hoc analysis.