Fig. 10.

β-FNAs effect on LPS-induced CXCL10, CCL2, and TNF-α expression in the liver of male and female C57BL/6J mice. Mice (n = 11–12/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CXCL10 (A), CCL2 (B), and TNF-α (C) of tissue homogenates were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment (p < 0.001) and sex (p < 0.0001) on CXCL10 levels in the liver; but no significant effect on interaction (p = 0.61). Two-way ANOVA determined CCL2 had a significant main effect of treatment (p < 0.0001), but not of sex (p = 0.25) or interaction (p = 0.28). Two-way ANOVA determined TNF-α showed no main effects of sex (p = 0.63), treatment (p = 0.41), or interaction (p = 0.99). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS