Fig. 14.

β-FNAs effect on LPS-induced NFκB-p65 expression in the spleen and liver of male and female C57BL/6J mice. Mice (n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of NFκB-p65 of spleen A and liver B of tissue homogenates were measured by western blot analysis (representative western blots are shown above each area analyzed). Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of sex (p < 0.0001) on levels of NFκB-p65 in the spleen; but no significant effect of treatment (p = 0.16), nor a significant interaction (p = 0.62). In the liver two-way ANOVA determined levels of NFκB-p65 had a significant main effect of sex (p < 0.0001) but no significant effect of treatment (p = 0.28), nor a significant interaction (p = 0.26). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS