Fig. 7.

β-FNAs effect on LPS-induced NF-κB-p65 expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice (n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of NF-κB-p65 of whole brain (A), hippocampus (B), prefrontal cortex (C), and cerebellum/brain stem (D) of tissue homogenates were measured by western blot analysis (representative western blots are shown above each area analyzed). Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment (p < 0.02) and sex (p < 0.0001) on levels of NF-κB-p65 in the whole brain; but no significant effect of interaction (p = 0.74). Two-way ANOVA determined NF-κB-p65 in the hippocampus had a significant main effect of sex (p < 0.01), treatment (p < 0.001), and interaction (p < 0.03). In the prefrontal cortex two-way ANOVA determined levels of NF-κB-p65 had a significant main effect of sex (p < 0.0001), but not treatment (p = 0.66) or interaction (p = 0.68). Two-way ANOVA determined in the cerebellum/brain stem that NF-κB-p65 had a no significant main effect of sex (p = 0.42), treatment (p = 0.08), or interaction (p = 0.62). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS