Fig. 9.

β-FNAs effect on LPS-induced CXCL10, CCL2, and TNF-α expression in the spleen of male and female C57BL/6J mice. Mice (n = 11–12/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CXCL10 (A), CCL2 (B), and TNF-α (C) of tissue homogenates were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment (p < 0.001), sex (p < 0.0001), and interaction (p < 0.001) on CXCL10 levels in the spleen. Two-way ANOVA determined CCL2 had a significant main effect of sex (p < 0.0001), treatment (p < 0.0001), and interaction (p < 0.0001). Two-way ANOVA determined TNF-α had a significant main effect of treatment (p < 0.003), but not sex (p = 0.25) or interaction (p = 0.54). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS