(a) Flow cytometry analysis of RAW 264.7 cells
incubated with 100
μg/mL R-BSA@PSiNPs, 100 μg/mL BSA@PSiNPs, or 100 μg/mL
PSiNPs for 12 h after the staining of JC-1 probes; (b) respiratory
chain activity, (c) mitochondrial ROS, and (d) cytosolic ROS production
of RAW 264.7 cells treated with 100 μg/mL R-BSA@PSiNPs, 100
μg/mL BSA@PSiNPs, or 100 μg/mL PSiNPs for 12 h. Untreated
RAW 264.7 cells as a control and ones stimulated by LPS + IFN-γ
as a positive control (n = 4 biological independent
samples). Error bars are based on standard errors of the mean (***P < 0.001 or *P < 0.05 by ANOVA with
Tukey’s post-test).