Figure 3.

M [6]a methylation and methylation-associated factors upregulated in BMSCs during osteogenic differentiation.(a) M⁶A content in BMSCs with or without the induction for osteogenic differentiation was determined. (b) Relative mRNA expression of METTL3, METTL14, FTO and ALKBH in BMSCs with or without the induction for osteogenic differentiation was measured via qRT-PCR. β-actin was the internal reference. (c-e) Relative METTL3 mRNA (c) and protein (d-e) expression in BMSCs on day 1, 3, 7 and 14 post osteogenic differentiation was further measured with qRT-PCR and Western blot. β-actin was the internal reference. All experiments have been performed independently in triplicate and data were expressed as mean ± standard deviation (SD). *p<0.05, **p<0.01, ***p<0.001, vs. 0 d. M⁶a: N⁶-Methyladenosine; METTL3: methyltransferase like 3, METTL14: methyltransferase like 14, FTO: FTO Alpha-Ketoglutarate Dependent Dioxygenase, ALKBH1: AlkB Homolog 1.