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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2023 Jan 9;460:116375. doi: 10.1016/j.taap.2023.116375

Fig. 3.

Fig. 3.

Binding activity of Keap1-Nrf2 PPI inhibitors to estrogen receptor (ER). PolarScreen™ Estrogen Receptor Alpha Competitor Assay kit was obtained from Life Technologies. A total assay volume of 20 μL was used. Estradiol and test compounds (0, 1, 10, 100 μM) were added to the appropriate wells of the assay plate. The plate was incubated at room temperature for 2 hours. Fluorescence polarization value (mP) was calculated based on the parallel and perpendicular fluorescence intensity (F∥ and F⊥) with respect to the linearly polarized excitation light.