FIGURE 4.

Effect of molecules identified in the pharmacological screening on Cystathionine beta synthase (CBS), cystathionine γ lyase (CSE) and NQO1 mRNA and CBS protein levels. (A–C) The level of CBS (A), CSE (B), and NQO1 mRNAs: three genes involved in the defense against oxidative stress and which are regulated by the NF-κB and the Akt/GSK3β pathways, was assessed by RT-qPCR 24 h after treatment with 30 μM of 9-methylstreptimidone, 150 nM of daunorubicin hydrochloride, 10 nM of 8α-hydroxy-verrucarin A (VA-8), 200 nM of chrysomycin (Chrys) and 50 μM of NHX in HepG2 cells. (D) Similarly, the level of CBS protein was assessed by western-blot 24 and 48 h after treatment with the same drugs. Ratios of CBS/α-tubulin signals are indicated below each lane for 48 h fater treatment. (A–C) Comparison of each condition with DMSO, one-way ANOVA with Dunnett’s post-hoc test: **p < 0.01; ****p < 0.0001. Note that there is no error bar for DMSO because the different values we obtained were expressed as percentages compared to the DMSO condition which was set up at 100%.