Table 1. List of primers used in this study.
| Target locus ID | Gene product, Gene symbol | Primer ID | Sequence (5’– 3’) | Source |
|---|---|---|---|---|
| Primers for use with Bacillus velezensis E68 | ||||
| GYA98_RS07520$ | Multifunctional protein RecA | recA-F | AAAAAACAAAGTCGCTCCTCCG | [42] |
| recA | recA-R | CGATATCCAGTTCAGTTCCAAG | ||
| GYA98_RS15380$ | Protein translocase subunit SecA | oNL25_secA_f | CGTTTAAAGTGCAGCTGATGG | This paper |
| secA | oNL26_secA_r | GGCTCGCCAGATATTCGTTG | ||
| GYA98_RS15140 | Heme response regulator HssR | oNL45_hssR_F | TATGAAGGTGACGAGCGTAC | This paper |
| hssR | oNL46_hssR_R | TGGATGGCAAACGGATAGTC | ||
| GYA98_RS00430 | Alkaline phosphatase D | oNL47_phoD_F | GCGTTTGAAGTGAATGCCG | This paper |
| phoD | oNL48_phoD_R | CAGAGAATGACGCTGTCAGAC | ||
| GYA98_RS07565 | Spore coat protein E | oNL49_cotE_F | ACCAACACGATCTCACCTGG | This paper |
| cotE | oNL50_cotE_R | TCGGCGTAAGAGTACCAGAC | ||
| GYA98_RS00125 | ECF RNA polymerase sigma factor SigW | oNL53_sigW_F | GCCTGACAAGTACAGAACGG | This paper |
| sigW | oNL54_sigW_R | CTGCCTCTGTGTATCCTTGTC | ||
| GYA98_RS03850 | ECF RNA polymerase sigma factor SigM | oNL67_sigM_F | GACCACAGCAAAGTAAAGCC | This paper |
| sigM | oNL68_sigM_R | ATACCCTGTCAGCACTTCC | ||
| GYA98_RS15180 | ATP phosphoribosyltransferase | oNL69_hisG_F | AGTTATTTCAGAGAGCAGGGC | This paper |
| hisG | oNL70_hisG_R | CTACGATTCTGTCAGCAAGCC | ||
| GYA98_RS03165 | Catechol-2,3-dioxygenase | oNL71_catE_F | GTCATCTCGCAGACAGATCG | This paper |
| catE | oNL72_catE_R | GGTCAGGCAGAAGTATCGC | ||
| GYA98_RS12240 | Citrate synthase 2 | oNL73_citZ_F | TGAGATGTCAATCCGTGTCG | This paper |
| citZ | oNL74_citZ_R | GATCGGCGTGAACAGATCG | ||
| Primers for use with Fusarium graminearum DAOMC 180378 | ||||
| FGRAMPH1_01G03049# | Ubiquitin thiolesterase | oNL29_GzUBH-F | CTCGAGGCCAGCAAAAAGTCA | [43] |
| UBH | oNL30_GzUBH-R | ATCGCCGTTAGGGGTGTCTG | ||
| FGRAMPH1_01G26865# | Tubulin beta chain | oNL43_TUB2-F | GTTGATCTCCAAGATCCGTG | [44] |
| TUB2 | oNL44_TUB2-R | CATGCAAATGTCGTAGAGGG | ||
| FGRAMPH1_01G01687 | Pleiotropic ABC efflux transporter of multiple drugs YBT1 | oNL55_ybt1_F | GCTACGCCTGCAAATGAACC | This paper |
| YBT1 | oNL56_ybt1_R | CTTAGGTCGAGCTACAATGGC | ||
| FGRAMPH1_01G15627 | ZEB2-regulated ABC transporter 1 | oNL57_zra1_F | GCTTCCAGGACATGAACGTC | This paper |
| ZRA1 | oNL58_zra1_R | GTCAATTCTGCGCTTGTTGG | ||
| FGRAMPH1_01G00143 | Nonribosomal peptide synthetase GRA1 | oNL59_gra1_F | ACTTGGAAGCTTTCTACCTGG | This paper |
| GRA1 | oNL60_gra1_R | CGTTGATCTATCACCAGCGAG | ||
| FGRAMPH1_01G05661 | Acetylesterase | oNL61_aes1_F | TGGTCAAGTCGATCAACCTC | This paper |
| AES1 | oNL62_aes1_R | CAGTGGGATTCTTGATTGCC | ||
| FGRAMPH1_01G12231 | D-malate dehydrogenase | oNL63_dmlA_F | GGTAATGACGAACCTGAGCC | This paper |
| DmlA | oNL64_dmlA_R | CGATGGTGACTGCATGAAGG | ||
| FGRAMPH1_01G05741 | Mitochondrial aldehyde dehydrogenase | oNL65_aldh_F | GGAGGAAATCTTTGGACCGG | This paper |
| ALDH2B4 | oNL66_aldh_R | GAGTTGAGCTTCTGAGTGACC | ||
| FGRAMPH1_01G19889 | RuvB-like helicase 2 | oNL75_RVB2_F | TTCGCAACTTGTCTCTGCC | This paper |
| RVB2 | oNL76_RVB2_R | GGCTACAAACTTGATGCTGC | ||
| FGRAMPH1_01G06539 | DNA repair protein | oNL77_RAD5_F | AGTTCACATCTTTCCTCAGCC | This paper |
| RAD5 | oNL78_RAD5_R | CGTTAAGTACAGCGGCTCG | ||
$Housekeeping genes for Bacillus velezensis E68.
#Housekeeping genes for F. graminearum DAOMC 180378