Table 6. Normalized expression of core biosynthetic genes from biosynthetic gene clusters encoding secondary metabolites in B. velezensis E68.
Compound | Core biosynthetic gene locus ID | TPM$ in single culture | TPM in dual culture |
---|---|---|---|
Surfactin | GYA98_RS00790 | 41.62444 | 54.49393 |
GYA98_RS00795 | 39.72677 | 36.2239 | |
GYA98_RS00800 | 10.51762 | 14.11884 | |
GYA98_RS00810 | 49.16629 | 56.89863 | |
GYA98_RS00815 | 55.97234 | 52.11947 | |
GYA98_RS00820 | 77.41527 | 64.22825 | |
Macrolactin H | GYA98_RS06300 | 237.6562 | 90.06079 |
GYA98_RS06305 | 521.1573 | 100.0418 | |
GYA98_RS06310 | 527.2992 | 96.8625 | |
GYA98_RS06315 | 622.4262 | 113.8966 | |
GYA98_RS06320 | 783.2996 | 181.6299 | |
GYA98_RS06325 | 440.9877 | 118.3611 | |
GYA98_RS06330 | 744.2543 | 223.779 | |
Bacillaene | GYA98_RS07605 | 410.8624 | 488.6164 |
GYA98_RS07610 | 420.911 | 373.5302 | |
GYA98_RS07615 | 558.4976 | 478.7145 | |
GYA98_RS07625 | 660.5088 | 360.3584 | |
GYA98_RS07640 | 1183.568 | 361.8864 | |
GYA98_RS07645 | 1198.974 | 260.8726 | |
GYA98_RS07650 | 792.9114 | 160.4905 | |
GYA98_RS07655 | 943.0376 | 233.3925 | |
GYA98_RS07660 | 1074.126 | 305.5386 | |
Bacillomycin D | GYA98_RS08205 | 345.918 | 221.1193 |
GYA98_RS08210 | 604.1638 | 437.0655 | |
GYA98_RS08215 | 565.6975 | 475.9636 | |
GYA98_RS08220 | 277.014 | 215.3259 | |
Fengycin | GYA98_RS08300 | 3.060367 | 33.54516 |
GYA98_RS08315 | 2.122462 | 36.92852 | |
GYA98_RS08335 | 571.6845 | 177.9275 | |
GYA98_RS08340 | 543.9846 | 173.68 | |
GYA98_RS08345 | 693.3596 | 238.2508 | |
GYA98_RS08350 | 463.5909 | 187.7093 | |
GYA98_RS08355 | 580.417 | 294.5991 | |
GYA98_RS08365 | 281.5708 | 150.397 | |
Difficidin | GYA98_RS10060 | 1267.013 | 857.4785 |
GYA98_RS10065 | 1026.037 | 719.9703 | |
GYA98_RS10070 | 899.1106 | 563.8603 | |
GYA98_RS10075 | 1083.195 | 696.7137 | |
GYA98_RS10080 | 901.9802 | 558.2665 | |
GYA98_RS10085 | 912.1075 | 526.2694 | |
GYA98_RS10090 | 1367.896 | 835.8435 | |
GYA98_RS10095 | 1072.906 | 647.6522 | |
GYA98_RS10100 | 1126.655 | 623.7472 | |
GYA98_RS10105 | 510.1765 | 295.7482 | |
GYA98_RS10110 | 953.4498 | 515.7316 | |
GYA98_RS10135 | 573.8718 | 259.0871 | |
Bacillibactin | GYA98_RS13635 | 159.2003 | 49.03839 |
GYA98_RS13645 | 259.1896 | 116.4149 | |
Amylocyclicin | GYA98_RS13745 | 25433.92 | 14390.07 |
Bacilysin | GYA98_RS16580 | 1691.767 | 989.5184 |
Genes were identified as core biosynthetic genes by antiSMASH.
$transcripts per million (TPM)