TABLE 1.
Straina | Allele | Azocasein units/OD unit | Hemagglutination titer |
---|---|---|---|
C6706 | crp+ | 9.36 ± 0.20 | 64 |
KSK394 | crp | 0.28 ± 0.07 | >2 |
C6709-1 | rpoS+ | 7.45 ± 0.30 | 128 |
DSM-V491 | ΔrpoS | 2.07 ± 0.27 | 64–128 |
Three independent single-colony cultures of each strains were diluted 1,000-fold in 50 ml of TSB and incubated with shaking (200 rpm) at 30°C to an OD595 of >4 to ensure complete derepression of Hap in each wild-type strain.