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. 2023 Jan 12;15:1006125. doi: 10.3389/fnmol.2022.1006125

Figure 2.

Figure 2

The excitability of PKCδCeA and SOMCeA neurons is increased in CFA 1D mice. (A) Schematic of AAV-DIO-mCherry injected into the SOM-Cre mice. (B) Representative images of AAV-DIO-mCherry expression in the SOM-Cre mice. Scale bar, 40 μm. (C) The mCherry-labeled neurons within the CeA were colocalized with the SOM immunofluorescence. Scale bar, 20 μm. (D,E) Sample traces (D) and statistical data (E) for action potential firing recorded from SOMCeA neurons (red cells in C) in SOM-CFA 1D or SOM-Saline 1D mice (n = 23 cells from five mice for SOM-Saline 1D group; n = 22 cells from five mice for SOM-CFA 1D group; F(1, 43) = 4.807, p = 0.0338). (F) Summary of the rheobases of action potentials from SOMCeA neurons (red cells in C) in SOM-CFA 1D or SOM-Saline 1D mice (n = 23 cells from five mice for SOM-Saline 1D group; n = 22 cells from five mice for SOM-CFA 1D group; t43 = 2.603, p = 0.0126). (G) The resting membrane potential (Vrest) of the SOM-CFA 1D group increased compared with that of the saline group (n = 23 cells from five mice for the SOM-Saline 1D group; n = 22 cells from five mice for the SOM-CFA 1D group; t43 = 4.129, p = 0.0002). (H) Diagram of crossing PKCδ-Cre mice and Ai9 reporter mice. (I) Representative images of PKCδ neurons expressing tdTomato in the CeA from PKCδ-tdTOM mice. Scale bar, 40 μm. (J) PKCδ immunofluorescence were colocalized with tdTomato-labeled neurons within the CeA. Scale bar, 20 μm. (K,L) Sample traces (K) and statistical data (L) for action potential firing recorded from PKCδCeA neurons (red cells in J) in CFA 1D or Saline 1D PKCδ-tdTOM mice (n = 16 cells from four mice for per group; F(1, 30) = 10.22, p = 0.0033). (M) Summary of the rheobases of action potentials from PKCδCeA neurons (red cells in J) in CFA 1D or Saline 1D PKCδ-tdTOM mice (n = 16 cells from four mice for per group; t30 = 2.254, p = 0.0317). (N) The resting membrane potential (Vrest) of the PKCδ-CFA 1D group increased compared with that of the saline group (n = 16 cells from four mice for per group; t30 = 2.973, p = 0.0058). All data are shown as the mean ± SEM, * p < 0.05; ** p < 0.01 and *** p < 0.001. Two-way repeated-z measures ANOVA with Bonferroni post-hoc analysis for (E,L); unpaired t-test for (F,G,M,N).