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. 2023 Jan 12;15:1006125. doi: 10.3389/fnmol.2022.1006125

Figure 4.

Figure 4

The expression and function of ASIC1a in GABACeA neurons. (A) Representative images of GABAergic neurons expressing tdTomato in the CeA from GAD2-tdTOM mice. Scale bars, 200 μm. (B,C) Images (B) and statistics data (C) showing that ASIC1a immunofluorescence were colocalized with tdTomato-labeled neurons within the CeA (n = 6 slices from three mice). Scale bar, 40 μm. (D,E) Representative images (D) and quantification (E) of Western blots showing that CFA injection did not induce significant changes in the expression of total ASIC1a in CeA (E, Day 1: t10 = 1.28, p = 0.2295, n = 6 mice per group; Day 3: t8 = 2.064, p = 0.0729, n = 5 mice per group; Day 7: t8 = 0.9581, p = 0.3661, n = 5 mice per group). (F,G) Representative images (F) and quantification (G) of Western blots showing that the membrane content of ASIC1a in CeA were not changed in mice 1 day after CFA injection, compared with Saline 1D group (G, Saline 1D, n = 4 mice; CFA 1D, n = 5 mice; t7 = 1.93, p = 0.0949). (H) Representative images of AAV-DIO-mCherry expression in the SOM-Cre mice. Scale bar, 40 μm. (I,J) Images (I) and statistics data (J) showing that ASIC1a immunofluorescence were colocalized with mCherry-labeled neurons within the CeA (n = 6 slices from three mice). Scale bar, 40 μm. (K) Representative images of PKCδ neurons expressing tdTomato in the CeA from PKCδ-tdTOM mice. Scale bars, 40 μm. (L,M) Images (L) and statistics data (M) showing that ASIC1a immunofluorescence were colocalized with tdTomato-labeled neurons within the CeA (n = 6 slices from three mice). Scale bar, 40 μm. (N,O) Representative current traces (N) and summarized data (O) showing that the application of PcTx1 (100 μM) induced larger outward currents in the SOMCeA neurons in acute brain slices of CFA 1D mice (n = 6 cells from five mice for per group; t10 = 2.372, p = 0.0391). (P,Q) Representative current traces (P) and summarized data (Q) showing that the application of PcTx1 (100 μM) induced larger outward currents in the PKCδCeA neurons in acute brain slices of CFA 1D mice (n = 5 cells from five mice for per group; t8 = 2.660, p = 0.0288). All data are shown as the mean ± SEM, n.s., not significant; * p < 0.05. unpaired t-test for (E,G,O,Q).