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. 2023 Jan 12;15:1006125. doi: 10.3389/fnmol.2022.1006125

Figure 5.

Figure 5

Pharmacological manipulations of ASIC1a in the CeA did not affect normal sensation and mood. (A) Schematic of bilateral PcTx1 injection into the CeA of C57 mice. (B) Typical image of bilateral cannula injection sites. Scale bar, 500 μm. (C) An outline of the pharmacological experimental procedure. (D) The mechanical pain threshold did not change after CeA injection of PcTx1 in Saline 1D mice (Saline-ACSF, n = 7 mice; Saline-PcTx1, n = 6 mice; F(1,11) = 0.4129, p = 0.5337). (E,H) Heatmaps showing the locomotion traces of Saline-ACSF mice and Saline-PcTx1 mice in the open field test (OFT) and elevated plus maze test (EPMT). (F,G,I) Summarized data showing no difference between ACSF and PcTx1-treated groups in OFT and EMPT in Saline 1D mice (F, n = 8 mice per group in OFT; entries in center in OFT, t14 = 0.5869, p = 0.5666; time in center in OFT, t14 = 0.3806, p = 0.7092; G, total distances in OFT, t14 = 1.504, p = 0.1549; I, n = 6 mice per group in EPMT; entries in the open arms in EPMT, t10 = 0.5207, p = 0.6139; time in the open arms in EPMT, t10 = 0.5167, p = 0.6166). (J,K) Sample traces (J) and statistical data (K) for action potential firing recorded from GABACeA neurons in Saline 1D mice treated with ACSF or PcTx1 (n = 16 cells from four mice for per group; F(1,30) = 0.1375, p = 0.7134). (L) Summary of the rheobases of action potentials from Saline 1D mice treated with ACSF or PcTx1 (n = 16 cells from four mice for per group; t30 = 2.294, p = 0.029) (M) The resting membrane potential (Vrest) showing no difference between Saline-ACSF mice and Saline-PcTx1 mice (n = 16 cells from four mice for per group; t30 = 1.947, p = 0.0610). All data are shown as the mean ± SEM, n.s., not significant; * p < 0.05. Two-way repeated-measures ANOVA with Bonferroni post-hoc analysis for (D,K); unpaired t-test for (F,G,I,L,M).