Fig. 5.

Knockdown of HIF-1α downregulated miR-155–5p and alleviated ER stress and fibrosis in OTA-exposed HK-2 cells. Cells were transfected using siRNA control (si-NC) or siHIF-1α followed by exposure of 100 nM OTA for 48 h. A: miR-155–5p levels. B: mRNA levels of HIF-1α, fibrosis, and ER stress markers. C: protein levels of HIF-1α, fibrosis, and ER stress markers. Significant difference between control and the test group was expressed using Student’s t-test, * p < 0.05, ** p < 0.01, and *** p < 0.001.