FIG. 2.
Detection of anti-KMP11 IgG antibody levels in the sera of mice immunized with DNA plasmids or saline solution. BALB/c (top panel) and C57BL/6-A2.1/Kb (bottom panel) mice were immunized intramuscularly four times with saline solution (◊) or 100 μg of each the DNA vectors pCMV4 (□), pCMV4.11 (▵), and pCMV4.11.70 (●). Production of IgG antibodies to KMP11 was evaluated by ELISA (29) on days 0, 21, 42, 56, 63, 77, 91, 105, 119, and 126 using 1 μg of recombinant KMP11 protein/well. Data are optical density (OD) values of pooled sera from six mice per group. These and all subsequent data show representative results of at least three independent experiments. Asterisks indicate immunization days.