FIG. 3.
IgG isotype level generated against KMP11 protein in mice immunized with pCMV4.11.70. The antibody level was determined by ELISA with sera from BALB/c (top panel) and C57BL/6-A2.1/Kb (bottom panel) mice, intramuscularly immunized with pCMV4.11.70 DNA, using 1 μg of recombinant KMP11 protein/well. IgG1 (○) and IgG2a (●) antibodies produced against KMP11 were evaluated in serum samples obtained on days 0, 21, 42, 56, 63, 77, 91, 105, 119, and 126. Data are optical density (OD) values of pooled sera from 6 mice. Asterisks indicate immunization days.