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. 2023 Jan 26;14(1):60. doi: 10.1038/s41419-023-05600-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A List of top USP49-associated proteins identified by mass spectrometry analysis. HEK-293T cells were co-transfected with GFP, GFP-USP49Long, or GFP-USP49Short in combination with RPN1-FLAG (B) or USP44-FLAG (C). Cell lysates were then subjected to immunoprecipitation with control IgG or anti-FLAG antibodies. D HEK-293T transduced with USP49Long^WT, USP49Short^WT, USP49Long^W158A, and USP49Short^W158A, together with CETN1 (upper panel), CETN2 (middle panel) or CETN3 (lower panel) were subjected to immunoprecipitation and immunoblotted using the indicated antibodies.