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. 2023 Jan 26;14:413. doi: 10.1038/s41467-023-36004-2

Fig. 1. Development of functional d12fABEs with high activities and diversified scopes.

Fig. 1

a Diagram showing Cas12f domain organization and internal fusing sites (red lines) for the generation of potential miniABEs. b Heatmaps from left to right showing A-to-G editing frequencies of d12fABEs-2-8e and d12fABEs-8e in blue gradient color. Various potential d12fABEs are generated by fusing TadA-8e monomer (hereafter 8e), dimer (hereafter 2-8e) to N-, C-terminus or internal sites of d12f. Editing activities were evaluated against sgRNA 12f-sg89. c A-to-G editing frequencies at each adenine position across 12 endogenous sites were quantified and summarized for N-/CL-/DS128-/DS130-d12fABE-8e (106 W) in blue, orange, violet, and green lines, respectively. d Heatmap showing editing activities of d12fABEs-TadA* and d12fABEs-TadA* (V82G) against sgRNA 12f-sg89 in blue gradient color. e A-to-G editing frequencies at each adenine position across 12 endogenous sites were quantified and summarized for N-dRRAABE-TadA* (82 G) and CL-dRRAABE-TadA* (82G) in purple and cyan lines, respectively. NC negative control. All data were collected from three independent experiments and presented as mean ± SEM. in histograms. Source data are provided as a Source Data file.