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. 2023 Jan 13;13:1065495. doi: 10.3389/fimmu.2022.1065495

Figure 3.

Figure 3

Frequency of Vδ2 cells as a percentage of live cells was assessed by flow cytometry following 14 days stimulation with IL-2 alone or IL-2 with ZA, BCG or HK-BCG. N=10-39, **p<0.005, ****p<0.0001, non-parametric analysis of variance with Tukey’s post hoc for multiple pairwise comparisons (A). Expression of NK associated activatory markers (B) and inhibitory checkpoint receptors (C) was determined on Vγ9Vδ2 T-cells in PBMC stimulated for 14 days with IL-2 alone, ZA or BCG, both with IL-2, using flow cytometry. N=10, *p<0.05, **p<0.005, ***p<0.0005, ****p<0.0001, non-parametric mixed effects analysis with Tukey’s post hoc for multiple pairwise comparisons. Expression of activatory and inhibitory receptors was determined in Vγ9Vδ2 T-cells from 6 donors at baseline (D0) and following stimulation for 14 days with IL-2 alone (IL2), ZA (ZA) or BCG (BCG), both with IL-2, using RNAseq (D).