Table 5.
Concentrations of Gelsemium alkaloids in pig spinal cord and bile after 45 d of consecutive administered G. elegans powder at a dose of 2% per kilogram feed (μg/kg) (n = 5).
| Compounds | Spinalcord (μg/kg) | Bile (μg/kg) | |||||
|---|---|---|---|---|---|---|---|
| 6h | 1d | 3d | 6h | 1d | 3d | 7d | |
| GA-1 | 5.3 ± 3.1 (5.2 ± 2.7) | 1.4 ± 0.3 (ND) | 1.1 ± 0 (ND) | ND | ND | ND | ND |
| GA-2 (*koumine) |
11.6 ± 7.6 (17.2 ± 14.1) |
7.3 ± 1.7 (ND) |
ND (ND) |
ND (22.9 ± 13.0) |
ND (1.6 ± 1.7) |
ND (ND) |
ND (ND) |
| GA-4 (*gelsemine) |
ND | ND | ND | ND | ND | ND | ND |
| GA-5 | 4.2 ± 2.0 (3.2 ± 1.5) |
ND (ND) |
ND (ND) |
2.9 ± 0.0 (110.1 ± 80.6) |
8.5 ± 4.8 (4.4 ± 3.0) |
ND (ND) |
ND (ND) |
| GA-6 (*gelsenicine) |
ND | ND | ND | 1.3 ± 0.0 | ND | ND | ND |
| GA-7 | 6.5 ± 3.7 | ND | ND | ND (15.0 ± 4.3) |
ND (1.9 ± 1.2) |
ND (ND) |
ND (ND) |
| GA-9 | 98.3 ± 71.6 | 25.9 ± 19.1 | 1.1 ± 0.9 | 3.0 ± 4.2 | 3.9 ± 2.6 | 2.6 ± 1.3 | ND |
| GA-10 | 18.0 ± 9.9 | ND | ND | ND | ND | ND | ND |
| GA-11 | 1.3 ± 0.7 | ND | ND | 6.5 ± 11.1 | 13.0 ± 10.8 | 2.4 ± 1.1 | ND |
| GA-12 | ND | ND | ND | 83.3 ± 0.0 | 121.4 ± 89.5 | 15.8 ± 17.0 | 16.0 ± 0.0 |
| GA-14 | 2.8 ± 1.6 | 1.1 ± 0.4 | ND | ND | ND | ND | ND |
| GA-15 | ND | ND | ND | 3.4 ± 0.0 | 7.5 ± 2.9 | ND | ND |
| GA-16 | 84.8 ± 57.2 | 17.4 ± 14.5 | ND | ND | ND | ND | ND |
| GA-17 | ND | ND | ND | ND | ND | ND | ND |
| GA-19 | 2.4 ± 2.2 | 1.0 ± 0.3 | ND | ND | ND | ND | ND |
| GA-20 | 1.4 ± 0.9 | ND | ND | 67.7 ± 0.0 | 85.7 ± 65.1 | 7.7 ± 7.9 | 5.6 ± 0.0 |
| GA-21 | 1.4 ± 1.2 | ND | ND | 1.4 ± 0.0 | 1.0 ± 0.5 | ND | ND |
| GA-22 | 5.3 ± 2.8 | 2.4 ± 0.8 | ND | 1.7 ± 2.3 | 3.2 ± 21 | ND | ND |
| GA-23 | ND | ND | ND | 7.3 ± 0.0 | 12.2 ± 10.4 | ND | ND |
| GA-24/25 | 1.0 ± 0.9 | ND | ND | ND | ND | ND | ND |
| GA-27 | 5.3 ± 3.1 (5.2 ± 2.7) |
1.4 ± 0.3 (ND) |
1.1 ± 0 (ND) |
4.9 ± 8.4 | 10.9 ± 5.2 | 6.2 ± 5.6 | 4.7 ± 3.9 |
*
, represents results of the precise quantitative method; ND, not detected.