Figure 4.

The responses of enriched CD4⁺ T-cell from immunized cattle to different stimuli were measured in cattle from immunogenicity studies. (A) The p67C-specific proliferative cellular response; and (B) the p67C-specific IFNγ-secreting cells per million of CD4⁺ T-cells in individual animals from group 1 to 3. Two different stimuli were used: soluble protein (sol. p67C) and a pool of 25-mer p67C overlapping peptides (p67C peptides). Negative stimulus responses are also included: ovalbumin and irrelevant peptides (irr. peptides). In both panels individual animals (n=3), the mean and standard error of the mean bars are shown.