FIG. 7.

Cytokine production in vitro is altered in γ/δ TCR KO mice. Plastic-adherent peritoneal cells harvested at various times from mice injected i.p. with 1.2 × 10⁴ CFU of Listeria were restimulated in vitro, and cytokines in culture supernatants were analyzed by ELISA. IFN-γ (A) was measured after stimulation for 96 h with rIL-12 (5 ng/ml) and IL-18 (50 ng/ml). IL-12p40 (B) and IL-10 (C) were measured after incubation for 24 h with HKLM (10⁷/ml). In previous experiments, these stimuli and incubation conditions were found to be optimal for each of these cytokines. (D and E) Frequencies of F4/80⁺ cell subsets as determined by flow cytometry.