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Active ATLG binding assay. (A) Schematic representation of flow cytometry analysis to measure active ATLG. Serum from ATLG-treated patients is incubated with healthy donor-derived leukocytes. Active ATLG is detected by FITC-conjugated swine anti-rabbit antibody on CD45+ cells, CD3+, and CD3+/CD4+ lymphocytes. (B) Calibration curve [mean fluorescent intensity vs. ATLG concentration, (μg/ml)] on CD45+ cells, CD3+ and CD3+/CD4+ lymphocytes.
