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. 2023 Jan 13;13:1089369. doi: 10.3389/fimmu.2022.1089369

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Copyright © 2023 Kang, Lee, Kim, Lee, Lee, Cho, Oh, Kim, Park, Han, Kim, Kim, Yoon, Doh, Chung, Hong, Choi and Kim

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Engineering and establishment of α-Cot-NK92 cells as a universal CAR system (A) The conventional and universal CAR-NK system. (B) Levels of surface expression of CAR in NK92 or α-Cot-NK92 cells with/without α-HER2-Cot. The α-Cot-NK92 cells were cultured with puromycin for 3 d after α-Cot-CAR lentiviral transduction. Subsequently, transgene expression was evaluated using flow cytometry analysis (FACS) with α-Myc-PE (upper) and α-human IgG-PE conjugated antibody (hIgG, lower) after incubation with/without α-HER2-Cot. CAR, chimeric antigen receptor; Cot, cotinine; NK, natural killer.