TABLE 1.
Strains and plasmids
| Species | Strain ID | Description | References |
|---|---|---|---|
| Cloning | |||
| Escherichia coli | IM08B | Used For Cloning (DH10B Δdcm with hsdS CC8-1of NRS384) | Monk et al. (2015) |
| Background | |||
| Staphylococcus aureus | ALC7589 | SH1000 (Functional rsbU derivative of 8325-4 rsbU+) | Horsburgh et al. (2002) |
| ALC4495 | SH1000 containing empty xylose-inducible vector, pEPSA5 | This Study | |
| ALC2727 | SH1000 containing promoterless pALC1484:gfp vector | Kahl et al. (2000) | |
| ALC9379 | SH1000 containing pALC1484:spn promoter-gfp | This Study | |
| Teg49 mutants | |||
| Staphylococcus aureus | ALC8744 | Teg49 mutant of SH1000; chromosomal deletion of 70-bp Teg49 from nt 305 to 375 from the ATG start codon of the sarA gene | This Study |
| ALC9377 | Teg49 genomic complement (ALC8744 restored with wild type Teg49) | This Study | |
| ALC8789 | ALC8744 containing an empty xylose-inducible vector, pEPSA5 | This Study | |
| ALC8790 | ALC8744 containing pEPSA5 carrying 196-bp Teg49 fragment DNA under a xylose-inducible promoter | This Study | |
| ALC9380 | Teg49 mutant (ALC8744) containing pALC1484:spn promoter-gfp | This Study | |
| ALC7289 | SH1000 Teg49 HP1 loop mutation of Teg49 (ATTGCGC→CGCTATA) | Kim et al. (2014) | |
| ALC7288 | SH1000 Teg49 HP1 stem mutation of Teg49 | Kim et al. (2014) | |
| ALC7290 | SH1000 Teg49 HP2 loop mutation of Teg49 (GTCGATT→TATCGGC) | Kim et al. (2014) | |
| ALC7291 | SH1000 Teg49 HP2 stem mutation of Teg49 | Kim et al. (2014) | |
| ALC8842 | SH1000 Teg49 HP3 Loop Mutant (ACTAAACCAAATGCTAA→GGTAGTTTTAAAAATAA) | This Study | |
| ALC9005 | SH1000 Teg49 HP3 stem mutation of Teg49 | This Study | |
| sarA mutants | |||
| Staphylococcus aureus | ALC2732 | SH1000 ΔsarA::ermC | Ballal et al. (2010) |
| ALC9378 | SH1000 sarA nonsense mutant (ATG→TAATAA) | This Study | |
| ALC8373 | SH1000 sarA P3 promoter scrambles | Manna et al. (2018) | |
| spn mutants | |||
| Staphylococcus aureus | ALC9381 | SH1000 spn deletion (382 bp genomic deletion, beginning 28 bp from the ATG start to 45 bp after the TAA stop codon) | This Study |
| ALC9382 | SH1000 spn genomic complement using pMAD | This Study | |
| ALC9383 | SH1000 with both Teg49 mutation and Δspn | This Study | |
| ALC9384 | spn synonymous mutant (TTC GCC GAT AAA to TTT TCA GAC AAG) (Amino Acid: F-A-D-N to F-A-D-N) | This Study | |
| ALC9385 | spn non-synonymous mutant (TTC GCC GAT AAA to TTT CTT CTA CGT) (Amino Acid: F-A-D-N to F-L-L-R) | This Study | |
| sae and rnc mutants | |||
| Staphylococcus aureus | ALC4473 | sae:Tn551 knockout isogenic for SH1000 | Xiong et al. (2006) |
| ALC8243 | Δrnc that is, RNase III genomic deletion of SH1000 with pMAD | Manna et al. (2018) | |
| ALC8445 | Δrnc genomic complement (ALC8243 restored with wild type rnc using pMAD) | Manna et al. (2018) | |
| pSK236 | CmR | Shuttle vector containing pUC19 cloned into the HindIII site of pC194 | This Study |
| pMAD | AmpR, ErmR | E. coli-S. aureus shuttle vector containing the temperature-sensitive origin of replication and bgaB | Arnaud et al. (2004) |
| pEPSA5 | AmpR, CmR | E. coli-S. aureus xylose-inducible shuttle vector containing xylose-inducible promoter for conditional expression of the desired gene | Forsyth et al. (2002) |
| pEPSA5::Teg49 | AmpR, CmR | pEPSA5 containing 196-bp Teg49 sRNA | Kim et al. (2014) |
| pALC1484 | AmpR, CmR | Modified pSK236 shuttle vector with a promoterless gfpuvr reporter gene preceded by an S. aureus ribosome binding site | Kahl et al. (2000) |