TABLE 2.
RNA-seq of ΔTeg49 mutant
| Genes | Fold change | p value | Corrected FDR | Pan genome ID | Annotation |
|---|---|---|---|---|---|
| SAOUHSC_01055a | 2.75 | 5.42E-15 | 2.36E-13 | SAUPAN003335000 | Membrane protein (A lot of genomes have a small product in that region, see SAUPAN003335000) |
| SAOUHSC_01014 | 2.72 | 7.62E-18 | 4.24E-16 | purF | Amidophosphoribosyltransferase |
| SAOUHSC_01016 | 2.61 | 4.78E-18 | 2.79E-16 | purN | Phosphoribosylglycinamide formyltransferase |
| SAOUHSC_01017 | 2.55 | 1.72E-16 | 8.94E-15 | purH | Bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase |
| SAOUHSC_01889 | 2.5 | 4.33E-20 | 2.72E-18 | ribD | Riboflavin biosynthesis protein RibD |
| SAOUHSC_01015 | 2.44 | 1.61E-14 | 6.87E-13 | purM | Phosphoribosylaminoimidazole synthetase |
| SAOUHSC_01887 | 2.33 | 6.84E-16 | 3.21E-14 | ribA | Riboflavin biosynthesis protein |
| SAOUHSC_03042 | 2.33 | 6.67E-13 | 2.53E-11 | SAUPAN006469000 | Integrase/recombinase core subunit |
| SAOUHSC_01888 | 2.31 | 2.30E-21 | 1.66E-19 | ribB | Riboflavin synthase subunit alpha |
| SAOUHSC_01018 | 2.24 | 2.73E-13 | 1.09E-11 | purD | Phosphoribosylamine--glycine ligase |
| SAOUHSC_00401 | 2.16 | 1.44E-21 | 1.08E-19 | SAUPAN002132000 | Myeloperoxidase inhibitor SPIN |
| SAOUHSC_01330 | 2.03 | 1.67E-20 | 1.14E-18 | guaC | Guanosine 5′-monophosphate oxidoreductase |
| SAOUHSC_00473 | 0.49 | 6.85E-11 | 2.37E-09 | SAUPAN002241000 | Small ORF only in NCTC8325 chromosome, between prsA and rplY |
| SAOUHSC_01403 | 0.48 | 1.32E-17 | 7.20E-16 | cspA | Cold shock protein |
| SAOUHSC_02681 | 0.47 | 5.92E-13 | 2.28E-11 | narG | Nitrate reductase subunit alpha |
| SAOUHSC_02671 | 0.47 | 5.00E-27 | 5.44E-25 | narK | Nitrite extrusion protein |
| SAOUHSC_02426 | 0.46 | 2.48E-16 | 1.26E-14 | YjiH | Membrane protein, nucleoside recognition domain (YjiH-Like) |
| SAOUHSC_00898 | 0.46 | 6.85E-17 | 3.64E-15 | argH | Argininosuccinate lyase |
| SAOUHSC_01275 | 0.45 | 2.16E-15 | 9.94E-14 | glpF | Aquaporin family protein (glycerol) |
| SAOUHSC_02130 | 0.39 | 2.15E-18 | 1.28E-16 | yebG | Hypothetical protein with NETI domain (YebG-like, DNA damage sensing) |
| SAOUHSC_00899 | 0.35 | 3.08E-23 | 2.95E-21 | argG | Argininosuccinate synthase |
| SAOUHSC_02662 | 0.29 | 3.80E-16 | 1.90E-14 | scrA | PTS system sucrose-specific transporter subunit IIBC |
Notes: RNA-Seq performed with wild type Staphylococcus aureus SH1000, a Teg49 mutant, and an inducible pEPSA:Teg49 complemented strain. It shows 22 genes differentially regulated by the Teg49 mutant but not the SH1000 isogenic parental strain. Gene numbers, given according to SH1000/NCTC8325 designation, fold change, statistical values, and Aureowiki gene information, are shown. Aureowiki annotations are based on NCBI RefSeq analyzed at (https://aureowiki.med.uni-greifswald.de). Paired-end sequencing was analyzed using CLC and edgeR. Significantly regulated genes were selected for 2× fold change, and a p-value of <.001. Genes regulated by SarA, ribosomal genes, phage, and transposon genes were excluded from the analysis.