Skip to main content
. 2023 Jan 13;13:1089987. doi: 10.3389/fimmu.2022.1089987

Figure 1.

Figure 1

NOD mice fed with anti-inflammatory diet were protected from autoimmune diabetes. (A) 4-weeks-old female NOD mice were fed with anti-inflammatory diet (AID) or standard (STD) diet up to 32 weeks of age (end of the experiment). In the AID the total amount of fats was 13,3% (3,6% of fish oil, 2% of linseed oil and 7% of corn oil), with PUFA ω3:ω6 ratio of 1:3 (20000mg/kg ω3 and 60000mg/kg ω6), and 8% of fibers (100% Inulin). STD diet contained 5% of fats (100% corn oil) with PUFA ω3:ω6 ratio of 1:190 (150 mg/kg ω3 and 28500 mg/kg ω6) and 5% of fibers (100% Cellulose). NFE, nitrogen-free extract (B) Incidence of autoimmune diabetes in NOD mice fed with AID (n=25) and STD diet (n=20). (C) Diabetic mice from each group (AID or STD diet-fed) were sacrificed after two consecutive measurements of hyperglycemia (> 250mg/dl), while diabetes resistant mice were sacrificed at the end of the experiment (32 weeks of age). Hematoxylin and eosin staining of pancreatic tissues was performed to detect lymphocyte infiltrates. On the left: one representative image of pancreatic tissue showing islet infiltration (in a diabetic NOD mouse fed with STD diet) or intact islets (in a diabetes-resistant NOD mice fed with AID diet) are shown. On the right: percentages of pancreatic islets infiltrated, peri-infiltrated with lymphocytes or intact (no infiltrates) out of total analyzed islets in Hematoxylin and Eosin-stained pancreata of NOD mice fed with AID or STD diet. Five randomly selected sections were analyzed from each mouse (n=6 mice per group). *p < 0.05.