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. 2023 Jan 13;13:1089987. doi: 10.3389/fimmu.2022.1089987

Figure 2.

Figure 2

AID restores gut barrier integrity in NOD mice. (A) RT-qPCR analysis of claudin 1 (Cldn1), tight junction protein 1 (Tjp1) and Occludin (Ocln) on tissue homogenates from the intestine of NOD mice fed with anti-inflammatory (AID) or standard (STD) diet at 14 weeks of age (n=8 per group). (B) RT-qPCR analysis of Muc1, Muc2, Muc3, and Muc4 mucin genes in the intestine of NOD mice fed with AID or STD diet (n=8 per group). (C) Alcian Blue staining of colon sections (20X) of NOD mice fed with AID or STD diet. The arrows on the apical side of the intestine (long arrow) indicate the thickness of the mucus layer while the smaller arrows along the crypts point to GC (stained in Blue). The percentage of GC in the two groups (right panel) was calculated within the total number of epithelial cells on randomly selected sections (n=5 per group, 6 sections/mouse). All data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ****p < 0.0001.