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. 2023 Jan 13;13:1089987. doi: 10.3389/fimmu.2022.1089987

Figure 3.

Figure 3

AID reduces intestinal inflammation and promotes gut immune homeostasis in NOD mice. (A) RT-qPCR analysis of cytokine genes encoding interleukin-1β (Il1b), subunit p19 of IL-23 (Il23), and IL-17A (Il17a) on tissue homogenates from the intestine of NOD mice fed with anti-inflammatory (AID) or standard (STD) diet at 14 weeks of age (n=8 per group). (B) Representative flow cytometry plots (Left) and percentages (Right) of INF-γ+CD4+ (Th1 cells), IL-17+CD4+ (Th17 cells) and INF-γ+IL-17+CD4+ (DP Th1/17 cells) cells out of total CD4+ T cells in the small intestinal tissue of 12-week-old NOD mice fed with AID or STD diet (n=7-8 mice/group). (C) Representative flow cytometry plots (Left) and percentages (Right) of FoxP3+CD25+CD4+ (FoxP3+Treg cells) and IL-10+FOXP3-CD4+ (Tr1 cells) cells out of total CD4+ T cells in the small intestinal lamina propria of 12-week-old NOD mice fed with AID or STD diet (n=7-8 mice/group). All data are presented as mean percentages ± SEM. *p < 0.05; **p < 0.01.