FIG. 4.

Effect of 6-OHDA treatment on IFN-γ and TNF-α production induced by anti-CD3 MAb or HK-LM in spleen cell cultures. Mice were injected intraperitoneally with 6-OHDA (solid bars) or the vehicle (open bars). Spleen cells were stimulated with anti-CD3 MAb or HK-LM for 24 h. The titers of IFN-γ (A) and TNF-α (B) in the culture supernatants were measured. Each result is the mean ± standard deviation based on samples obtained from three mice. An asterisk indicates that a value is significantly different from the value obtained for the vehicle-treated mice (P < 0.05). The results were reproduced in three repeated experiments.