FIG. 6.

Effect of 6-OHDA treatment on activation of PEC or splenic macrophages (sMΦ) obtained from 6-OHDA-treated mice (solid symbols) or vehicle-treated mice (open symbols). PEC (circles) or splenic macrophages (triangles) were mixed with L. monocytogenes cells and were cultured for 30 min at 37°C. After washing to remove the extracellular bacterial cells, the numbers of viable bacterial cells in the cell lysates at zero time and 2, 4, and 6 h later (A) and nitric oxide production by PEC and splenic macrophages in culture supernatants after 24 h of incubation (B) were estimated. Each result is the mean ± standard deviation based on four samples. The results were reproduced in three repeated experiments.