Figure 7.

The antioxidant activity of the predictive biomarker pipecolic acid

Impact of pipecolic acid on ROS production induced by Rosup (A) or H₂O₂ (B) in Eca109 and KYSE150 cells. The influence of pipecolic acid on the expression of the DNA damage marker γ-H2AX induced by Rosup (C) or H₂O₂ (D) in Eca109 and KYSE150 cells, respectively. The influence of pipecolic acid on the production of the DNA oxidative damage marker 8-oxo-dG induced by Rosup (E) or H₂O₂ (F) in Eca109 and KYSE150 cells, respectively. The impact of pipecolic acid on the proliferation and PCNA expression of Eca109 and KYSE150 cells treated with Rosup (G) or H₂O₂ (H). Error bars represent mean ± SEM. *, P < 0.05; ^**, P < 0.01; ^***, P < 0.001; ns, no significance (two-tailed Student’s t-test). Pipe, pipecolic acid; ROS, reactive oxygen species; 8-oxo-dG, 8-oxo-7,8-dihydro-20-deoxyguanosine; PCNA, proliferating cell nuclear antigen.