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. 2023 Jan 27;44(7):1455–1463. doi: 10.1038/s41401-022-01043-w

Fig. 1. Selection of antibody cocktails from the SARS-CoV-2 neutralizing antibody repertoire.

Fig. 1

a Epitope mapping of the antibody repertoire using a competition ELISA. A heatmap was used to show the competition percentages between two antibodies. The epitopes of antibodies were roughly classified into three groups according to their competitive relations. b Binding to SARS-CoV-1 S protein by antibodies in the repertoire. Trimeric SARS-CoV-1 S protein was coated on 96-well ELISA plates and incubated with 0.1 μg/mL of neutralizing antibodies. The binding was detected using a HRP-labeled goat anti-human IgG (Fc specific) antibody. c Concentration-dependent neutralization of SARS-CoV-1 pseudovirus by neutralizing antibodies. Serial diluted antibodies were incubated with the virus and used to infect 293T-ACE2 cells. The infection was quantified using a fluorescence quantification kit. Data from two replicates are shown as mean ± S.D. d Antibodies competitively blocked the ACE2 binding to SARS-CoV-2 S trimer as measured by ELISA. Recombinant human ACE2 protein and phosphate buffer solution (PBS) were used as controls.