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. 2023 Jan 27;13:1512. doi: 10.1038/s41598-023-28790-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cross-reactivity of anti-N20 Abs with different upper respiratory tract viruses such as Epstein-Barr Virus (EBV), Influenza A virus (IVA), Influenza B virus (IVB), respiratory syncytial virus (RSV), human Norovirus (hNoV) in comparison to positive controls – N protein, full-length protein N, and SARS-CoV 2 VLPs. Each well was coated with 100 ng of each sample (protein/VLPs/ inactivated virus); Anti-N20 antibodies were used in 1:1000 dilution. The nucleocapsid protein from the NL63 coronavirus was used as a negative control.