TABLE 1.

Oligonucleotide sequences of primers employed in RT-PCR analysis

Gene	Accession no.	Primer sequence and positionsa
b2 microglobulin	M17987	5′ 184-GAATTGCTATGTGTCTGGGT-203
		3′ 2300-CATCTTCAAACCTCCATGATG-2289
IL-1β	M15840	5′ 4336-GACACATGGGATAACGAGGC-4356
		3′ 5819-ACGCAGGACAGGTACAGATT-5800
IL-6	M54894	5′ 52-TGAACTCCTTCTCCACAAGC-71
		3′ 366-ATCCAGATTGGAAGCATCCA-347
IL-10	U16720	5′ 5511-ACCAAGACCCAGACATCAAG-5530
		3′ 7925-CAGGTACAATAAGGTTTCTCAAG-7947
IL-12 p40	AF180563	5′ 16-CAGCAGTTGGTCATCTCTTG-35,
		3′ 435-CCAGCAGGTGAAACGTCCA-416
IL-16	U82972	5′ 1683-ATGCCTGACCTCAACTCCTC-1703
		3′ 2030-CTCCTGATGACAATCGTGAC-2011
TNF-α	Z15026	5′ 578-TGAGCACTGAAAGCATGATC-597
		3′ 758-CCGCACCTCGACTCTCTATT-3739
TGF-β	M38449	5′ 301-GACAGGTTGGTAGCACGC-320
		3′ 394-GGACACCAACTATTGCTTCAG-414
IFN-γ	J00219	5′ 508-TCTGCATCGTTTTGGGTTCT-518
		3′ 2162-CAGCTTTTCGAAGTCATCTC-2142
ahpC	U18264	5′ 649-GCGATCAATTCCCCGCC-665
		3′ 1036-AAGGTCACGCGGTCGGC-1020
35-kDa antigen	M69187	5′ 669-GCGCACCCACCAAGCGC-685
		3′ 903-GACGCTCTGCTCGGCGG-887
MT10Sa	X60301	5′ 118-AGGGCCAGGTCGGTGGC-134
		3′ 470-AGATCCGGACGATCGGC-454
Ag85B	X62398	5′ 141-GGTCGAGTACCTGCAGG-157
		3′ 631-CGTCACCCATCGCGAGG-615
Ag85C	X57229	5′ 331-CCGCGTCGATGGGCCGC-347
		3′ 680-CAGCGCGGAACCGCCCG-664
ESAT6	X79652	5′ 24-GCAGTGGAATTTCGCGG-40
		3′ 247-CTTCGCTGATCGTCCGC-231
rpoB	U12205	5′ 665-CTCCGTACCCGGAGCGC-681
		3′ 1314-GCTCGCTGGTCCAGCCC-1298
invB	AF006054	5′ 1233-GGGGTGCCCTATTCGTGGGGT-1253
		3′ 1460-GCCGCCGCCTGGGCCGTAAAA-1439
sigF	U41061	5′ 171-GTTTGCCTGCCGGCTCACCGG-191
		3′ 715-CAACGGACGAAGCACCTCCCG-695
umaA2	U27357	5′ 11417-GTCGCAATGACTGGACCGCGG-11437
		3′ 11930-AAGCTGAACCTCGAACCCGGG-11909
eisb	AF144099	5′ -GGATCCGTCAGACCCACCGAGCAT-
		3′ -CGGATCCCCATCCATGGCGTGT-

^aEach primer sequence is indicated in its 5′-3′ direction. 

^bModified from Wei et al. (37).