Figure 1.

TIA1 Domain Structure and Fibril Seeding. (A) The TIA1 protein is composed of three RRM that precede a 96-residue LC domain. (B) TIA1 LC domain primary sequence in the single-letter amino acid code. The locations of missense mutations linked to neurodegenerative diseases are highlighted in red. The segment that forms the core of the seeded fibrils identified in this work is in bold letters. (C) Negatively stained TEM image of TIA1 LC domain fibrils prior to seeding. Arrows point to amorphous aggregates that form concomitantly with the fibrils. (D) A negatively stained TEM image of the seeded TIA1 LC domain fibrils shows a reduced number of amorphous aggregates.