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. 2023 Jan 13;145(3):1580–1592. doi: 10.1021/jacs.2c08596

Figure 3.

Figure 3

Liquid droplet aging of TIA1 LC domain wild-type and P362L mutant. (A) Bright field microscope images (BF) and negatively stained electron micrographs (TEM) of wild-type and P362L mutant TIA1 LC domain samples show the conversion of liquid droplets into amorphous aggregates that then convert into fibrils over the course of one week. (B) ThT fluorescence spectra recorded over the same time period show a slight increase in intensity after 1.5 h, which decreases at 4 h before increasing again over the course of a week. (C) Intrinsic Trp fluorescence spectra recorded over the same time period show a slight decrease in intensity and shift to lower wavelengths after 1.5 h, a trend that continues over the course of a week. In both (B) and (C), solid lines represent the data from wild-type TIA1 LC domain sample and dashed lines represent data from P362L mutant TIA1 LC domain sample. The error bars are the standard deviation of three measurements.