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. 2022 Dec 7;51(2):e12. doi: 10.1093/nar/gkac1136

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Gene-specific analysis of NAD-RNAs by ONE-seq. (A) Schematic workflow of gene-specific assessment of NAD modification with ONE-seq platform by qRT-PCR. ppp-RNA (106 nt) and NAD-RNA (106 nt) were included as a baseline negative control and a positive control, respectively. (B) Assessment of gene-specific NAD-capping by qRT-PCR. Based on ONE-seq platform, Cytochrome P450 family Cyp2c70 and Cyp3a11 involved in electron transport, Akr1c13 and Prxl2b of the metabolism-related genes, and Med17 and Ufc1 genes of gene regulatory pathways were examined. Total RNAs were from liver tissues of young mice (2-month).