Figure 2.

ORF112 Zα domain is essential and sufficient for CyHV-3 growth in cell culture and virulence in vivo. (A) Flowchart of the production of CyHV-3 ORF112 recombinants. Schematic representation of WT CyHV-3 BAC and derived CyHV-3 BAC ORF112 KO. The ability of different constructions to rescue viral growth after cotransfection with the CyHV-3 BAC ORF112 KO was tested. When viral growth was observed, recombinants were given a short name (in blue) to simplify the reading of the text. (B) Viral growth assay of the indicated CyHV-3 recombinants. Data are presented as the means ± the SEM of triplicate measurements. No statistical differences were detected. (C) Virulence in carp of the indicated CyHV-3 recombinants. No statistical differences were detected between the two viruses. PCR analyses performed on randomly selected dead fish confirmed the absence of cross-contamination.