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. 2022 Sep 22;51(2):806–830. doi: 10.1093/nar/gkac761

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — ORF112 and ORF112 Zα domain accumulate in RNP condensates in infected cells. (A) Cells infected with CyHV-3 recombinants expressing ORF112 (full-length protein, CyHV-3 ORF112) or ORF112 aa189–280 (Zα domain only, CyHV-3 ORF112 aa189–280) were fixed, permeabilized, and treated for double immunofluorescence staining of dsRNA (α-dsRNA, green signal) and ORF112 (α-ORF112, red signal). DNA was stained with Dapi (blue signal). Line intensity profiles are shown in the right panels. (B) Cells infected with CyHV-3 recombinants expressing ORF112 (full-length protein, left panels) or ORF112 aa189–280 (Zα domain only, right panels) were fixed, permeabilized and treated with the indicated enzymes before immunostaining of dsRNA (α-dsRNA, green signal) and ORF112 (α-ORF112, red signal). DNA was stained with Dapi (blue signal).