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. 2022 Sep 22;51(2):806–830. doi: 10.1093/nar/gkac761

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — CyHV-3 infected cells contain Z-RNA. Cells infected with CyHV-3 recombinants expressing ORF112 (full-length protein, CyHV-3 ORF112) or ORF112 aa189-280 (Zα domain only, CyHV-3 ORF112 aa189–280) were fixed and permeabilized. Cells were then mock-treated (untreated, two upper rows of panel) or treated with Proteinase K (two middle rows of panel) or treated with both Proteinase K and RNase III (two lower rows of panel) before double immunofluorescence staining of Z-RNA (α-Z-RNA, green signal) and ORF112 (α-ORF112, red signal). DNA was stained with Dapi (blue signal).