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. 2022 Sep 22;51(2):806–830. doi: 10.1093/nar/gkac761

Figure 8.

Figure 8.

Ability of ORF112 and its subdomains to mediate LLPS induced by PEG or Z-RNA. (A) Schematic representation of ORF112 recombinant forms tested. Black triangles illustrate mutation impairing Z-binding activity. (B) In vitro LLPS induced by incubation of the indicated mCherry N-terminal tagged recombinant forms of ORF112 (10 μM) with PEG-6000 (5%, v/v) (second column) or 10 μM of FAM-Z-RNA (three right columns). See also Supplementary Figures S2 and S3. (C) In vitro LLPS induced by incubation of the indicated mixture of mCherry (red) and GFP (green) N-terminal tagged recombinant forms of ORF112 (10 μM of each protein) with PEG-6000 (5%, v/v, three middle panels) or 10 μM of Z-RNA (three right panels). (D) Schematic representation of the hypothetical interactions leading to the LLPS results observed.