Figure 2.

Contribution of the AP to complement activation depends on antibody concentration. In ELISA setting, C3b deposition was measured on varying densities of heat agglutinated IgG (AHG) coat, with 25% (v/v) serum and antibodies to block the alternative or classical pathway, respectively. At high AHG densities, inhibiting the AP with anti‐FB or anti‐FD had no effect on C3b deposition. Bars show mean + SD of n = 3 independent experiments, statistical significance was tested using one‐way ANOVA with Dunnett's multiple comparisons test. **P < 0.01, ***P < 0.001, ****P < 0.0001.