Fig. 4.

Cytokine production and proliferation ability of CD26^neg/low/high CD8⁺ T cells in CLL. A Representative flow plots, (B) the frequency of TNF-$\alpha$, (C) IFN-$\gamma ,$ and (D) TNF-$\alpha$⁺IFN-$\gamma$⁺ cells among CD26^neg/low/high CCR7⁻CD8⁺ T cells. E Representative plots, (F) cumulative data showing the frequency of TNF-$\alpha$⁺, (G) IFN-$\gamma$⁺, and (H) TNF-$\alpha$⁺IFN-$\gamma$⁺ among CD26^neg/low/high CCR7⁻CD8⁺ T cells in unstimulated or stimulated with anti-CD3/CD28 (3 g$\mathrm{\mu }$/ml, 1 g$\mathrm{\mu }$/ml) and a cocktail of IL-18 + IL-12 + IL-15 (100 ng/ml of each). (I) Representative flow plots and, (J) cumulative data of the frequency of IL-2 expressing cells among CD26^neg/low/high CD8⁺ T cells in unstimulated (black color) versus 5 h after in-vitro stimulation with PMA/ionomycin cocktail (Biolegend, 2 ng/ml) in the presence of Brefeldin A (1 g$\mu$/ml). (K) Representative flow plots and, (L) cumulative data of the frequency of CFSE^low (proliferated) cells among CD26^neg/low/high CCR7⁻CD8⁺ T cells, unstimulated (black color) versus 72 h stimulation. (M) Representative flow plots, and (N) cumulative data showing the MFI for ROR $\gamma \delta$ in CD26^neg, CD26^low, and CD26^high CD8⁺ T cells in CLL. Error bars represent the median with an interquartile range. Each dot represents an individual human sample