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A Growth kinetics of recombinant P. pastoris X-33/pGAPZαA-LevB1SacB system. Bioreactor scale on BMY medium with two sucrose additions at 35 and 53 h. Reaction conditions: 28 °C, 200–1200 rpm, final volumen of 0.8 L and total sucrose concentration of 200 g/L supplied in two stages. B Sucrose and monosaccharides consumption along the culture. C GPC. Levan molecular weigth distribution of the final products compared to the 2nd sucrose addition
